Intronserter, an advanced online tool for design of intron containing transgenes

Algal Research, Volume 42

The Team of Bielefeld University has launched their new web tool “Intronserter” for the reliable and standardized design of intron-containing transgenes for robust transgene expression from the algal nuclear genome. Intronserter implements back-translation of input amino acid sequences with user-defined codon usage tables, removes undesired sequence elements, minimizes exon lengths by systematic intron insertions, and generates ready-to-synthesize DNA sequences for expression of any target protein. We hope to greatly facilitate the design of intron-containing constructs and to promote advanced and mature engineering efforts with algal as well as other host organisms.

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