Intronserter, an advanced online tool for design of intron containing transgenes
The Team of Bielefeld University has launched their new web tool “Intronserter” for the reliable and standardized design of intron-containing transgenes for robust transgene expression from the algal nuclear genome. Intronserter implements back-translation of input amino acid sequences with user-defined codon usage tables, removes undesired sequence elements, minimizes exon lengths by systematic intron insertions, and generates ready-to-synthesize DNA sequences for expression of any target protein. We hope to greatly facilitate the design of intron-containing constructs and to promote advanced and mature engineering efforts with algal as well as other host organisms.
Access to Intronserter: https://bibiserv.cebitec.uni-bielefeld.de/intronserter